RT Journal Article SR Electronic T1 Comparative phosphorylation map of Dishevelled3 (DVL3) JF bioRxiv FD Cold Spring Harbor Laboratory SP 621896 DO 10.1101/621896 A1 Kateřina Hanáková A1 Ondřej Bernatík A1 Petra Ovesná A1 Marek Kravec A1 Miroslav Micka A1 Matěj Rádsetoulal A1 David Potěšil A1 Lukáš Čajánek A1 Zbyněk Zdráhal A1 Vítězslav Bryja YR 2019 UL http://biorxiv.org/content/early/2019/05/02/621896.abstract AB In the presented study we analyze phosphorylation of human Dishevelled 3 (DVL3) induced by its previously reported (CK1ε, NEK2, PLK1, CK2α, RIPK4, PKCδ) and the newly identified (TTBK2, Aurora A) kinases. DVL3 contains 131 Ser/Thr whose phosphorylation generates complex barcodes underlying diverse DVL3 functions in Wnt pathways and other processes. We use quantitative mass spectrometry and via several complementary pipelines calculate site occupancies and quantify phosphorylation of >80 phosphorylated residues. In order to visualize the complex phosphorylation patterns, we design a novel visualization diagram, phosphoplot. Finally, we compare the individual sample and data processing approaches, identify their strengths and weaknesses. Subsequently, we verified a set of anti-phospho-DVL antibodies and were able to successfully confirm induction for several of the phosphorylation sites. From the biological point of view, our data represent an important reference point and a toolbox for further analysis of DVL functions and phosphorylation events that control them.One sentence summary The study provides a comprehensive comparison of the phosphorylation of DVL3 induced by eight Ser/Thr kinases and identifies phosphorylation signatures associated with individual kinases.