TY - JOUR T1 - Significant control of Zika infection in macaques depends on the elapsing time after dengue exposure JF - bioRxiv DO - 10.1101/625293 SP - 625293 AU - Crisanta Serrano-Collazo AU - Erick X. Pérez-Guzmán AU - Petraleigh Pantoja AU - Mariah A. Hassert AU - Idia V. Rodríguez AU - Luis Giavedoni AU - Vida Hodara AU - Laura Parodi AU - Lorna Cruz AU - Teresa Arana AU - Melween I. Martínez AU - Laura White AU - James D Brien AU - Aravinda de Silva AU - Amelia K. Pinto AU - Carlos A. Sariol Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/05/02/625293.abstract N2 - Zika virus (ZIKV) infection remains a public health concern with the recent epidemic infecting millions of people in the Americas. Prior exposure to a single serotype of dengue virus (DENV) predisposes individuals to severe disease upon secondary heterologous DENV infection. Here we are showing that the length of time between DENV/ZIKV infections has a qualitative impact on controlling ZIKV infection. We identified limited but significant differences in the magnitude of the early humoral immune response associated to a period of twelve months but not three months of DENV convalescence. However, their role limiting ZIKV replication is not conclusive. There were not evidences of in vivo antibody-dependent amplification of ZIKV by previous DENV immunity in any group. We also confirmed that the significant differences among groups are mediated by the pre-existence of a robust effector memory T cell and cytotoxic activity mainly mediated by CD4+ T cells. We demonstrated that exposure to ZIKV 12 months after DENV infection afford a high level of T cell-mediated cross-protection that was not observed at span of 3-months. These results suggest that there is a window of optimal cross-protection between ZIKV and DENV with significant consequences. These results have pivotal implication while interpreting ZIKV pathogenesis in flavivirus-experimented populations, diagnostic results interpretation and vaccine designs among others. ER -