PT  - JOURNAL ARTICLE
AU  - Natalya A. Blessing
AU  - Zhenzhen Wu
AU  - Sethu Madhavan
AU  - Myung K. Shin
AU  - Maarten Hoek
AU  - John R. Sedor
AU  - John F. O’Toole
AU  - Leslie A. Bruggeman
TI  - APOL1 is not expressed in proximal tubules and is not filtered
AID  - 10.1101/623918
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 623918
4099  - http://biorxiv.org/content/early/2019/05/02/623918.short
4100  - http://biorxiv.org/content/early/2019/05/02/623918.full
AB  - The kidney expression pattern of APOL1 was examined using both protein and mRNA in situ methods on APOL1 bacterial artificial chromosome transgenic mice, with and without proteinuria. APOL1 was detected in podocytes and endothelial cells of the kidney, but was not expressed in tubular epithelia, nor was plasma APOL1 protein filtered and reabsorbed by the proximal tubule. APOL1 expression in podocytes and endothelia should remain the focus for mechanistic studies of APOL1-mediated pathogenesis.