RT Journal Article
SR Electronic
T1 Predicting microbial relative growth in a mixed culture from growth curve data
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 022640
DO 10.1101/022640
A1 Ram, Yoav
A1 Dellus-Gur, Eynat
A1 Bibi, Maayan
A1 Obolski, Uri
A1 Berman, Judith
A1 Hadany, Lilach
YR 2017
UL http://biorxiv.org/content/early/2017/11/24/022640.abstract
AB Estimates of microbial fitness from growth curves are inaccurate. Rather, competition experiments are necessary for accurate estimation. But competition experiments require unique markers and are difficult to perform with isolates derived from a common ancestor or non-model organisms. Here we describe a new approach for predicting relative growth of microbes in a mixed culture utilizing mono- and mixed culture growth curve data. We validated this approach using growth curve and competition experiments with E. coli. Our approach provides an effective way to predict growth in a mixed culture and infer relative fitness. Furthermore, by integrating several growth phases, it provides an ecological interpretation for microbial fitness.