TY - JOUR T1 - Unfolding the hippocampus: an intrinsic coordinate system for subfield segmentations and quantitative mapping JF - bioRxiv DO - 10.1101/146878 SP - 146878 AU - Jordan DeKraker AU - Kayla M. Ferko AU - Jonathan C. Lau AU - Stefan Köhler AU - Ali R. Khan Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/11/24/146878.abstract N2 - The hippocampus, like the neocortex, has a morphological structure that is complex and variable in its folding pattern, especially in the hippocampal head. The current study presents a computational method to unfold hippocampal grey matter, with a particular focus on the hippocampal head where complexity is highest due to medial curving of the structure and the variable presence of digitations. This unfolding was performed on segmentations from high-resolution, T2-weighted 7T MRI data from 12 healthy participants and one surgical patient with epilepsy whose resected hippocampal tissue was used for histological validation. We traced a critical hippocampal component, the hippocampal sulcus and stratum radiatum, lacunosum moleculaire, (SRLM) in these images, then employed user-guided semi-automated techniques to detect and subsequently unfold the surrounding hippocampal grey matter. This unfolding was performed by solving Laplace’s equation in three dimensions of interest (long-axis, proximal-distal, and laminar). The resulting ‘unfolded coordinate space’ provides an intuitive way of mapping the hippocampal subfields in 2D space (long-axis and proximal-distal), such that similar borders can be applied in the head, body, and tail of the hippocampus independently of variability in folding. This unfolded coordinate space was employed to map intracortical myelin and thickness in relation to subfield borders, which revealed intracortical myelin differences that closely follow the subfield borders used here. Examination of a histological sample from a patient with epilepsy reveals that our unfolded coordinate system shows biological validity, and that subfield segmentations applied in this space are able to capture features not seen in manual tracing protocols.Research highlightsSRLM in hippocampal head consistently detected with 7T, T2 isotropic MRIHippocampal grey matter unfolded using Laplace’s equation in 3DIntracortical myelin and thickness mapped in unfolded coordinate spaceUnfolded subfields capture critical structural regularities and agree with histology ER -