@article {Huang625533, author = {Yao-Shen Huang and Ching-Hsiang Fan and Ning Hsu and Chun-Yao Wu and Chu-Yuan Chang and Shi-Rong Hong and Ya-Chu Chang and Anthony Yan-Tang Wu and Vanessa Guo and Yueh-Chen Chiang and Wei-Chia Hsu and Nai-Hua Chiu and Linyi Chen and Charles Pin-Kuang Lai and Chih-Kuang Yeh and Yu-Chun Lin}, title = {Sonogenetic modulation of cellular activities using an engineered auditory-sensing protein}, elocation-id = {625533}, year = {2019}, doi = {10.1101/625533}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Biomolecules that respond to different external stimuli enable the remote control of genetically modified cells. Chemogenetics and optogenetics, two tools that can control cellular activities via synthetic chemicals or photons, respectively, have been widely used to elucidate underlying physiological processes. These methods are, however, very invasive, have poor penetrability, or low spatiotemporal precision, attributes that hinder their use in therapeutic applications. We report herein a sonogenetic approach that can manipulate target cell activities by focused ultrasound stimulation. This system requires an ultrasound-responsive protein derived from an engineered auditory-sensing protein prestin. Heterogeneous expression of mouse prestin containing two parallel amino acid substitutions, N7T and N308S, that frequently exist in prestins from echolocating species endowed transfected mammalian cells with the ability to sense ultrasound. An ultrasound pulse of low frequency and low pressure efficiently evoked cellular calcium responses after transfecting with prestin(N7T, N308S). Moreover, pulsed ultrasound can also non-invasively stimulate target neurons expressing prestin(N7T, N308S) in deep regions of mice brains. Our study delineates how an engineered auditory-sensing protein can cause mammalian cells to sense ultrasound stimulation. Moreover, owing to the great penetration of low-frequency ultrasound (\~{}400 mm in depth), our sonogenetic tools will serve as new strategies for non-invasive therapy in deep tissues of large animals like primates.}, URL = {https://www.biorxiv.org/content/early/2019/05/03/625533}, eprint = {https://www.biorxiv.org/content/early/2019/05/03/625533.full.pdf}, journal = {bioRxiv} }