PT  - JOURNAL ARTICLE
AU  - Sebastian Virreira Winter
AU  - Florian Meier
AU  - Christoph Wichmann
AU  - Juergen Cox
AU  - Matthias Mann
AU  - Felix Meissner
TI  - EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification
AID  - 10.1101/225649
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 225649
4099  - http://biorxiv.org/content/early/2017/11/27/225649.short
4100  - http://biorxiv.org/content/early/2017/11/27/225649.full
AB  - We developed EASI-tag (Easily Abstractable Sulfoxide-based Isobaric tag), a new generation of amine-derivatizing and sulfoxide-containing isobaric labelling reagents, which dissociate at low collision energy and generate peptide-coupled, interference-free reporter ions with high yield. Efficient isolation of 12C precursors and quantification at the MS2 level enable accurate determination of quantitative differences between multiplexed samples. EASI-tag makes isobaric labeling applicable to any bottom up proteomics workflow and to benchtop mass spectrometers.