PT  - JOURNAL ARTICLE
AU  - Mikhail Repin
AU  - Sergey Pampou
AU  - David J. Brenner
AU  - Guy Garty
TI  - The use of a centrifuge-free RABiT-II system for high-throughput micronucleus analysis
AID  - 10.1101/627596
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 627596
4099  - http://biorxiv.org/content/early/2019/05/04/627596.short
4100  - http://biorxiv.org/content/early/2019/05/04/627596.full
AB  - The cytokinesis-block micronucleus (CBMN) assay is considered as the most suitable biodosimetry method for automation. Previously, we automated this assay on a commercial robotic biotech high-throughput system (RABiT-II) adopting both a traditional and an accelerated micronucleus protocol, both using centrifugation steps for lymphocyte harvesting and washing, after whole blood culturing. Here we describe further development of our accelerated CBMN assay protocol for using on High Throughput/High Content Screening (HTS/HCS) robotic systems without a centrifuge. This opens the way for implementation of the CBMN assay on a wider range of commercial automated HTS/HCS systems and thus increases the potential capacity of dose estimates following a mass-casualty radiological event.