RT Journal Article
SR Electronic
T1 The use of a centrifuge-free RABiT-II system for high-throughput micronucleus analysis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 627596
DO 10.1101/627596
A1 Mikhail Repin
A1 Sergey Pampou
A1 David J. Brenner
A1 Guy Garty
YR 2019
UL http://biorxiv.org/content/early/2019/05/04/627596.abstract
AB The cytokinesis-block micronucleus (CBMN) assay is considered as the most suitable biodosimetry method for automation. Previously, we automated this assay on a commercial robotic biotech high-throughput system (RABiT-II) adopting both a traditional and an accelerated micronucleus protocol, both using centrifugation steps for lymphocyte harvesting and washing, after whole blood culturing. Here we describe further development of our accelerated CBMN assay protocol for using on High Throughput/High Content Screening (HTS/HCS) robotic systems without a centrifuge. This opens the way for implementation of the CBMN assay on a wider range of commercial automated HTS/HCS systems and thus increases the potential capacity of dose estimates following a mass-casualty radiological event.