RT Journal Article
SR Electronic
T1 Rice blast resistance gene <em>Pii</em> is controlled by a pair of NBS-LRR genes <em>Pii-1</em> and <em>Pii-2</em>
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 227132
DO 10.1101/227132
A1 Hiroki Takagi
A1 Akira Abe
A1 Aiko Uemura
A1 Kaori Oikawa
A1 Hiroe Utsushi
A1 Hiroki Yaegashi
A1 Hideko Kikuchi
A1 Motoki Shimizu
A1 Yoshiko Abe
A1 Hiroyuki Kanzaki
A1 Hiromasa Saito
A1 Ryohei Terauchi
A1 Koki Fujisaki
YR 2017
UL http://biorxiv.org/content/early/2017/11/30/227132.abstract
AB Nucleotide-binding, leucine-rich repeat receptors (NLRs) are conserved cytosolic receptors that recognize pathogen effectors and trigger immunity in plants. Recent studies indicate that NLRs function in pairs. Rice resistance gene Pii has been known to confer resistance against rice blast pathogen Magnaporthe oryzae carrying AVR-Pii. Previously we reported isolation of Pii gene from the rice cultivar Hitomebore (Takagi et al. 2013). To further understand rice components required for Pii-mediated resistance, we screened 5,600 mutant lines of Hitomebore cultivar and identified two mutants that lost Pii resistance without any changes in Pii gene sequence. Application of MutMap-Gap, the whole genome sequencing-based method of mutation identification, to the two mutants revealed that they have mutations in another NLR gene located close to Pii. The F1 plants derived from a cross of the two mutants showed pii phenotype, demonstrating that the newly identified NLR gene is indeed a component of Pii resistance. We thus designate the previously isolated Pii gene as Pii-1 and the newly isolated NLR gene as Pii-2.