PT  - JOURNAL ARTICLE
AU  - Matthias Meurer
AU  - Yuanqiang Duan
AU  - Ehud Sass
AU  - Ilia Kats
AU  - Konrad Herbst
AU  - Benjamin C Buchmuller
AU  - Verena Dederer
AU  - Florian Huber
AU  - Daniel Kirrmaier
AU  - Martin Štefl
AU  - Koen Van Laer
AU  - Tobias P Dick
AU  - Marius K Lemberg
AU  - Anton Khmelinskii
AU  - Emmanuel D Levy
AU  - Michael Knop
TI  - A genome-wide resource for high-throughput genomic tagging of yeast ORFs
AID  - 10.1101/226811
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 226811
4099  - http://biorxiv.org/content/early/2017/11/30/226811.short
4100  - http://biorxiv.org/content/early/2017/11/30/226811.full
AB  - Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces cerevisiae ORFs. It consists of 5661 strains with an acceptor module inserted after each ORF, which can be efficiently replaced with tags or regulatory elements. We validate the library with targeted sequencing and demonstrate its use by tagging the yeast proteome with bright fluorescent proteins, determining how sequences downstream of ORFs influence protein expression and localizing previously undetected proteins.