PT  - JOURNAL ARTICLE
AU  - Bernett Lee
AU  - Samantha Cyrill
AU  - Wendy Lee
AU  - Rosella Melchiotti
AU  - Anand Andiappan
AU  - Michael Poidinger
AU  - Olaf Rötzschke
TI  - Analysis of archaic human haplotypes suggest 5-hmC to act as epigenetic guide for meiotic point recombination
AID  - 10.1101/227702
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 227702
4099  - http://biorxiv.org/content/early/2017/12/02/227702.short
4100  - http://biorxiv.org/content/early/2017/12/02/227702.full
AB  - Meiotic “point recombination” refers to homologue recombination events affecting only individual SNPs. Driven mostly by gene conversion, it is common process that allows for a gradual adaptation and maturation of haplotypes during genetic evolution. In contrast to crossover recombination it is not tied to predetermined recombination sites and therefore assumed to occur largely randomly. Our analysis of archaic human haplotypes however revealed striking differences in the local point recombination rate. A linkage-study of 1.9 million SNPs defined by the sequence of denisovan hominids revealed low rates in introns and quiescent intergenic regions but high rates in splice sites, exons, 5’- and 3’-UTRs, and CpG islands. Correlations with ChIP-Seq tracks from ENCODE and other public sources identified a number of epigenetic modifications, that associated directly with these recombination events. A particularly tight association was observed for 5-hydroxymethylcytosine marks (5hmC). The mark was enriched in virtually all of the functional regions associated with elevated point recombination rates, including CpG islands and ‘poised’ bivalent regions. As intermediate of oxidative demethylation, 5hmC is also a marker of recently opened gene loci. The data, thus, supports a model of ‘guided’ evolution, in which point recombination is directed by 5hmC marks towards the functionally relevant regions.5hmC5-hydroxymethylcytosine5mC5-methylcytosineBivFlankBivalent flanking regionbpBase-pairsCGICpG IslandsChIP-seqChromatin Immunoprecipitation and SequencingCOCrossoverDDRDNA damage repairDenisovan hDenisovan hominidDMC1Meiotic recombination protein, RecA homologENCODEEncyclopedia of DNA elementsEnhBivBivalent enhancerfaAbsolute frequency of derived allelesFitConsFitness consequenceGWAVAGenome-wide annotation of variantsH. sapiensHomo sapienshapCore haplotypeHLAHuman Leukocyte AntigenkbKilo-basepairsLDLinkage disequilibriumLWKLuhya from Webuye, KenyaMHCMajor Histocompatibility ComplexNCONon-CrossoverPRC2Polycomb repressive complex 2ReprPCPolycomb Repressed RegionsReprPCWkWeak Polycomb Repressed RegionsSNPSingle Nucleotide PolymorphismTEITransgenerational Epigenetic InheritenceTSSTranscriptional start siteTssBivBivalent transcriptional start siteTxTranscribed RegionTxFlankFlanking a transcribed regionTxWkWeakly transcribed regionUTRUntranslated RegionZNFZinc Finger