@article {Bayas228122, author = {Camille A. Bayas and Jiarui Wang and Marissa K. Lee and Jared M. Schrader and Lucy Shapiro and W.E. Moerner}, title = {Spatial organization and dynamics of RNase E and ribosomes in Caulobacter crescentus}, elocation-id = {228122}, year = {2017}, doi = {10.1101/228122}, publisher = {Cold Spring Harbor Laboratory}, abstract = {We report the dynamic spatial organization of Caulobacter crescentus RNase E (RNA degradosome) and ribosomal protein L1 (ribosome) using 3D single particle tracking and super-resolution microscopy. RNase E formed clusters along the central axis of the cell, while weak clusters of ribosomal protein L1 were deployed throughout the cytoplasm. These results contrast with RNase E and ribosome distribution in E. coli, where RNase E co-localizes with the cytoplasmic membrane and ribosomes accumulate in polar nucleoid-free zones. For both RNase E and ribosomes in Caulobacter, we observed a decrease in confinement and clustering upon transcription inhibition and subsequent depletion of nascent RNA, suggesting that RNA substrate availability for processing, degradation, and translation facilitates confinement and clustering. Moreover, RNase E cluster positions correlate with the subcellular location of chromosomal loci of two highly transcribed ribosomal RNA genes, suggesting that RNase E{\textquoteright}s function in ribosomal RNA processing occurs at the site of rRNA synthesis. Thus, components of the RNA degradosome and ribosome assembly are spatiotemporally organized in Caulobacter, with chromosomal readout serving as the template for this organization.}, URL = {https://www.biorxiv.org/content/early/2017/12/03/228122}, eprint = {https://www.biorxiv.org/content/early/2017/12/03/228122.full.pdf}, journal = {bioRxiv} }