PT  - JOURNAL ARTICLE
AU  - Hua Zhou
AU  - Xiao Liang
AU  - Aipeng Liu
AU  - Dongmei Nong
AU  - Yaqing Qin
AU  - Lianxiang Chen
AU  - Na Kang
TI  - Correlation of IL-17 level in gingival crevicular fluid of orthodontically induced inflammatory root resorption
AID  - 10.1101/627059
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 627059
4099  - http://biorxiv.org/content/early/2019/05/05/627059.short
4100  - http://biorxiv.org/content/early/2019/05/05/627059.full
AB  - Objective To investigate IL-17 expression in orthodontic tooth movement and orthodontic nickel-titanium spring-induced inflammatory root resorption.Methods Orthodontic nickel-titanium springs were ligated between the bilateral maxillary first molar and the incisors of the rats to establish a rat model of orthodontic tooth movement (OTM), each rat was subjected to two cycles of near-GCF and peripheral blood serum collection before and after force application, and IL-17 levels in GCF and serum were measured quantitatively by ELISA. Morphological changes in periodontal tissue and root of the experimental dentine were evaluated by hematoxylin and eosin staining. Tartrate-resistant acid phosphatase staining and immunohistochemistry were used to determine the osteoclast number and expression changes in IL-17, receptor activator of nuclear factor kappa-B ligand (RANKL), and osteoprotegerin (OPG) in the periodontal tissues, respectively, on the pressure side of the experimental tooth.Results IL-17 was detected in GCF and serum. The pressure area exhibited alveolar bone resorption only at a force of 20g. Additionally, a force of 60g led to root resorption. IL-17, RANKL/OPG and osteoclast number showed similar trend that all expressed increasing high level at early stage, then significantly decreased from days 5 to 14, and revealed 60g group the highest expression level while 0g group the lowest.The change in the IL-17 level in the GCF was strongly correlated with IL-17 and RANKL/OPG expression levels and osteoclast numbers in the periodontal ligament.Conclusions The results indicated that measuring IL-17 level in GCF can predict the risk of alveolar bone and root resorption induced by orthodontic treatment.OTMOrthodontic Tooth MovementInterleukin-17IL-17GCFGingival Crevicular FluidRANKLReceptor Activator of Nuclear factor Kappa-Β Ligand