PT  - JOURNAL ARTICLE
AU  - Stefania Bellini
AU  - Kelsey E. Fleming
AU  - Modhurika De
AU  - John P. McCauley
AU  - Maurice A. Petroccione
AU  - Lianna Y. D’Brant
AU  - Artem kachenko
AU  - SoYoung Kwon
AU  - Lindsey A. Jones
AU  - Annalisa Scimemi
TI  - Neuronal glutamate transporters control dopaminergic signaling and compulsive behaviors
AID  - 10.1101/224477
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 224477
4099  - http://biorxiv.org/content/early/2017/12/03/224477.short
4100  - http://biorxiv.org/content/early/2017/12/03/224477.full
AB  - There is an ongoing debate on the contribution of the neuronal glutamate transporter EAAC1 to the onset of compulsive behaviors. Here we use behavioral, electrophysiological, molecular and viral approaches in male and female mice to identify the molecular and cellular mechanisms by which EAAC1 controls the execution of repeated motor behaviors. Our findings show that in the striatum, a brain region implicated with movement execution, EAAC1 limits group I metabotropic glutamate receptor (mGluRI) activation, facilitates D1 dopamine receptor (D1R) expression and ensures long-term synaptic plasticity. Blocking mGluRI in slices from mice lacking EAAC1 restores D1R expression and synaptic plasticity. Conversely, activation of intracellular signaling pathways coupled to mGluRI in D1R-expressing striatal neurons of mice expressing EAAC1 leads to reduced D1R expression and increased stereotyped movement execution. These findings identify new molecular mechanisms by which EAAC1 can shape glutamatergic and dopaminergic signals and control repeated movement execution.SIGNIFICANCE STATEMENT Genetic studies implicate Slc1a1, a gene encoding the neuronal glutamate transporter EAAC1, with obsessive-compulsive disorder (OCD). EAAC1 is abundantly expressed in the striatum, a brain region that is hyperactive in OCD. What remains unknown is how EAAC1 shapes synaptic function in the striatum. Our findings show that EAAC1 limits activation of metabotropic glutamate receptors (mGluRI) in the striatum and, by doing so, it promotes D1R expression. Targeted activation of signaling cascades coupled to mGluRI in mice expressing EAAC1 reduces D1R expression and triggers repeated motor behaviors in mice. These findings provide new information on the molecular basis of OCD and suggest new avenues for its treatment.