RT Journal Article
SR Electronic
T1 A cellular and spatial map of the choroid plexus across brain ventricles and ages
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 627539
DO 10.1101/627539
A1 Neil Dani
A1 Rebecca H. Herbst
A1 Naomi Habib
A1 Joshua Head
A1 Danielle Dionne
A1 Lan Nguyen
A1 Cristin McCabe
A1 Jin Cui
A1 Frederick B. Shipley
A1 Ahram Jang
A1 Christopher Rodman
A1 Samantha J. Riesenfeld
A1 Feng Zhang
A1 Orit Rozenblatt-Rosen
A1 Aviv Regev
A1 Maria K. Lehtinen
YR 2019
UL http://biorxiv.org/content/early/2019/05/05/627539.abstract
AB The choroid plexus (ChP), located in each brain ventricle, produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier, but is under-characterized. Here, we combine single cell RNA-Seq and spatial mapping of RNA and proteins to construct an atlas of each ChP in the developing and adult mouse brain. Each ChP comprises of epithelial, endothelial, mesenchymal, immune, neuronal, and glial cells, with distinct subtypes, differentiation states and anatomical locations. Epithelial, fibroblast, and macrophage populations had ventricle-specific, regionalized gene expression programs across the developing brain. Key cell types are retained in adult, with loss of developmental signatures and maturation of ventricle-specific regionalization in the epithelial cells. Expression of cognate ligand-receptor pairs across cell subtypes suggests substantial cell-cell interactions within the ChP. Our atlas sheds new light on the development and function of the ChP brain barrier system, and will facilitate future studies on its role in brain development, homeostasis and disease.