PT  - JOURNAL ARTICLE
AU  - Cyrille L. Delley
AU  - Leqian Liu
AU  - Maen F. Sarhan
AU  - Adam R. Abate
TI  - Combined aptamer and transcriptome sequencing of single cells
AID  - 10.1101/228338
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 228338
4099  - http://biorxiv.org/content/early/2017/12/03/228338.short
4100  - http://biorxiv.org/content/early/2017/12/03/228338.full
AB  - The transcriptome and proteome encode distinct information that is important for characterizing heterogeneous biological systems. We demonstrate a method to simultaneously characterize the transcriptomes and proteomes of single cells at high throughput using aptamer probes and droplet-based single cell sequencing. With our method, we differentiate distinct cell types based on aptamer surface binding and gene expression patterns. Aptamers provide advantages over antibodies for single cell protein characterization, including rapid, in vitro, and high-purity generation via SELEX, and the ability to amplify and detect them with PCR and sequencing.