TY - JOUR T1 - Show me your neighbours, and I’ll tell you what you are – cellular microenvironment matters JF - bioRxiv DO - 10.1101/231282 SP - 231282 AU - Timea Toth AU - Tamas Balassa AU - Norbert Bara AU - Ferenc Kovacs AU - Andras Kriston AU - Csaba Molnar AU - Lajos Haracska AU - Farkas Sukosd AU - Peter Horvath Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/12/08/231282.abstract N2 - To answer major questions of cell biology, it is essential to understand cellular complexity. Modern automated microscopes produce vast amounts of images routinely, making manual analysis nearly impossible. Due to their efficiency, machine learning-based analysis software have become essential tools to perform single-cell-level phenotypic analysis of large imaging datasets. However, an important limitation of such methods is that they do not use the information gained from the cellular micro- and macroenvironment: the algorithmic decision is based solely on the local properties of the cell of interest. Here, we present how various microenvironmental features contribute to identifying a cell and how such additional information can improve single-cell-level phenotypic image analysis. The proposed methodology was tested for different sizes of Euclidean and nearest neighbour-based cellular environments both on tissue sections and cell cultures. Our experimental data verify that the microenvironment of a cell largely determines its entity. This effect was found to be especially strong for established tissues, while it was somewhat weaker in the case of cell cultures. Our analysis shows that combining local cellular features with the properties of the cell's microenvironment significantly improves the accuracy of machine learning-based phenotyping. ER -