RT Journal Article
SR Electronic
T1 Spotiton: New Features and Applications
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 230151
DO 10.1101/230151
A1 Venkata P. Dandey
A1 Hui Wei
A1 Zhening Zhang
A1 Yong Zi Tan
A1 Priyamvada Acharya
A1 Edward T. Eng
A1 William J. Rice
A1 Peter A. Kahn
A1 Clinton S. Potter
A1 Bridget Carragher
YR 2017
UL http://biorxiv.org/content/early/2017/12/08/230151.abstract
AB We present an update describing new features and applications of Spotiton, a novel instrument for vitrifying samples for cryoEM. We have used Spotiton to prepare several test specimens that can be reconstructed using routine single particle analysis to ~3 Å resolution, indicating that the process has no apparent deleterious effect on the sample integrity. The system is now in routine and continuous use in our lab and has been used to successfully vitrify a wide variety of samples.