PT  - JOURNAL ARTICLE
AU  - Bing Hou
AU  - Xinyi Liu
AU  - Dan Zhang
AU  - Zaifu Yang
AU  - Hui Hui
AU  - Peixin Wang
AU  - Jiarui Wang
AU  - Zilong Qiu
AU  - Yanyan Liu
AU  - Zhengyi Yang
AU  - Tianzi Jiang
TI  - Controlling emulsification for organic solvent-based tissue clearing
AID  - 10.1101/628925
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 628925
4099  - http://biorxiv.org/content/early/2019/05/07/628925.short
4100  - http://biorxiv.org/content/early/2019/05/07/628925.full
AB  - Organic solvent-based tissue clearing techniques are hampered by the quenching of fluorescent proteins, partially due to routine complete dehydration. Unexpectedly, we discovered that complete dehydration is unnecessary for organic solvents to clear tissues and that the hidden purpose has been to prevent emulsification. After controlling emulsification of organic solvent-cleared but incompletely dehydrated mouse brain, we achieved sufficient tissue transparency that allowed light-sheet imaging while well-preserving the fluorescence of fluorescent proteins.