TY - JOUR T1 - Controlling emulsification for organic solvent-based tissue clearing JF - bioRxiv DO - 10.1101/628925 SP - 628925 AU - Bing Hou AU - Xinyi Liu AU - Dan Zhang AU - Zaifu Yang AU - Hui Hui AU - Peixin Wang AU - Jiarui Wang AU - Zilong Qiu AU - Yanyan Liu AU - Zhengyi Yang AU - Tianzi Jiang Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/05/07/628925.abstract N2 - Organic solvent-based tissue clearing techniques are hampered by the quenching of fluorescent proteins, partially due to routine complete dehydration. Unexpectedly, we discovered that complete dehydration is unnecessary for organic solvents to clear tissues and that the hidden purpose has been to prevent emulsification. After controlling emulsification of organic solvent-cleared but incompletely dehydrated mouse brain, we achieved sufficient tissue transparency that allowed light-sheet imaging while well-preserving the fluorescence of fluorescent proteins. ER -