RT Journal Article
SR Electronic
T1 Synthetic peptide-induced internalization of biomolecules into various plant and algal cells via micropinocytosis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 630301
DO 10.1101/630301
A1 Jo-Ann Chuah
A1 Masaki Odahara
A1 Yutaka Kodama
A1 Takaaki Miyamoto
A1 Kousuke Tsuchiya
A1 Yoko Motoda
A1 Takanori Kigawa
A1 Keiji Numata
YR 2019
UL http://biorxiv.org/content/early/2019/05/07/630301.abstract
AB Efficient intracellular delivery of biomolecules is important for many different biological and biotechnological applications in living organisms, and is a prerequisite for certain types of fundamental and applied research. One major challenge is the delivery of unmodified, functional cargoes in a simple, time-efficient, and high-throughput manner. Herein, we present an efficient strategy that uses fusion peptides containing cell penetrating peptide, endosomal escape domain, and a sarcosine linker to introduce biomolecules, namely fluorescent protein and dextran, via macropinocytosis into the cells of various land plants and microalgae. Our peptide-mediated delivery system allows for high-throughput delivery of functional biomolecules within a few minutes to a few hours as well as open new possibilities for biology and biotechnology using difficult-to-transfect cell types.