@article {Kaletsky232728, author = {Rachel Kaletsky and Vicky Yao and April Williams and Alexi M. Runnels and Sean B. King and Alicja Tadych and Shiyi Zhou and Olga G. Troyanskaya and Coleen T. Murphy}, title = {Transcriptome Analysis of Adult C. elegans Cells Reveals Tissue-specific Gene and Isoform Expression}, elocation-id = {232728}, year = {2017}, doi = {10.1101/232728}, publisher = {Cold Spring Harbor Laboratory}, abstract = {The biology and behavior of adults differ substantially from those of developing animals, and cell-specific information is critical for deciphering the biology of multicellular animals. Thus, adult tissue-specific transcriptomic data are critical for understanding molecular mechanisms that control their phenotypes. We used adult cell-specific isolation to identify the transcriptomes of C. elegans{\textquoteright} four major tissues (or {\textquotedblleft}tissue-ome{\textquotedblright}), identifying ubiquitously expressed and tissue-specific {\textquotedblleft}super-enriched{\textquotedblright} genes. These data newly reveal the hypodermis{\textquoteright} metabolic character, suggest potential worm-human tissue orthologies, and identify tissue-specific changes in the Insulin/IGF-1 signaling pathway. Tissue-specific alternative splicing analysis identified a large set of collagen isoforms and a neuron-specific CREB isoform. Finally, we developed a machine learning-based prediction tool for 70 sub-tissue cell types, which we used to predict cellular expression differences in IIS/FOXO signaling, stage-specific TGF-b activity, and basal vs. memory-induced CREB transcription. Together, these data provide a rich resource for understanding the biology governing multicellular adult animals}, URL = {https://www.biorxiv.org/content/early/2017/12/12/232728}, eprint = {https://www.biorxiv.org/content/early/2017/12/12/232728.full.pdf}, journal = {bioRxiv} }