RT Journal Article SR Electronic T1 Transcriptome Analysis of Adult C. elegans Cells Reveals Tissue-specific Gene and Isoform Expression JF bioRxiv FD Cold Spring Harbor Laboratory SP 232728 DO 10.1101/232728 A1 Rachel Kaletsky A1 Vicky Yao A1 April Williams A1 Alexi M. Runnels A1 Sean B. King A1 Alicja Tadych A1 Shiyi Zhou A1 Olga G. Troyanskaya A1 Coleen T. Murphy YR 2017 UL http://biorxiv.org/content/early/2017/12/12/232728.abstract AB The biology and behavior of adults differ substantially from those of developing animals, and cell-specific information is critical for deciphering the biology of multicellular animals. Thus, adult tissue-specific transcriptomic data are critical for understanding molecular mechanisms that control their phenotypes. We used adult cell-specific isolation to identify the transcriptomes of C. elegans’ four major tissues (or “tissue-ome”), identifying ubiquitously expressed and tissue-specific “super-enriched” genes. These data newly reveal the hypodermis’ metabolic character, suggest potential worm-human tissue orthologies, and identify tissue-specific changes in the Insulin/IGF-1 signaling pathway. Tissue-specific alternative splicing analysis identified a large set of collagen isoforms and a neuron-specific CREB isoform. Finally, we developed a machine learning-based prediction tool for 70 sub-tissue cell types, which we used to predict cellular expression differences in IIS/FOXO signaling, stage-specific TGF-b activity, and basal vs. memory-induced CREB transcription. Together, these data provide a rich resource for understanding the biology governing multicellular adult animals