PT  - JOURNAL ARTICLE
AU  - Ronald D. Smith
AU  - Taliesin J. Kinser
AU  - Gregory D. Conradi Smith
AU  - Joshua R. Puzey
TI  - A likelihood ratio test for changes in homeolog expression bias
AID  - 10.1101/119438
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 119438
4099  - http://biorxiv.org/content/early/2017/12/13/119438.short
4100  - http://biorxiv.org/content/early/2017/12/13/119438.full
AB  - Background: Gene duplications are a major source of raw material for evolution and a likely contributor to the diversity of life on earth. Duplicate genes (i.e., homeologs, in the case of a whole genome duplication) may retain their ancestral function, sub- or neofunctionalize, or be lost entirely. A primary way that duplicate genes may evolve new functions is by altering their expression patterns. Comparing the expression patterns of duplicate genes may give clues as to whether any of these evolutionary processes have occurred.Results: We develop a likelihood ratio test for the analysis of the expression ratios of duplicate genes across two conditions (e.g., tissues). We demonstrate an application of this test by comparing homeolog expression patterns of 1,448 homeologous gene pairs using RNA-seq data generated from the leaves and petals of an allotetraploid monkeyflower (Mimulus luteus). We assess the sensitivity of this test to different levels of homeolog expression bias and compare the method to several alternatives.Conclusions: The likelihood ratio test derived here is a direct, transparent, and easily implemented method for detecting changes in homeolog expression bias that outperforms three alternative approaches. While our method was derived with homeolog analysis in mind, this method can be used to analyze changes in the ratio of expression levels between any two genes in any two conditions.