@article {Greenstein630970, author = {R.A. Greenstein and Ramon R. Barrales and Nicholas A. Sanchez and Jordan E. Bisanz and Sigurd Braun and Bassem Al-Sady}, title = {Set1/COMPASS repels heterochromatin invasion at euchromatic sites by disrupting Suv39/Clr4 activity and nucleosome stability}, elocation-id = {630970}, year = {2019}, doi = {10.1101/630970}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Protection of euchromatin from invasion by gene-repressive heterochromatin is critical for cellular health and viability. In addition to constitutive loci such as pericentromeres and subtelomeres, heterochromatin can be found interspersed in gene-rich euchromatin, where it regulates gene expression pertinent to cell fate. While hetero- and euchromatin are globally poised for mutual antagonism, the mechanisms underlying precise spatial encoding of heterochromatin containment within euchromatic sites remain opaque. We investigated ectopic heterochromatin invasion by manipulating the fission yeast mating type locus boundary, using a single-cell spreading reporter system. We found that heterochromatin repulsion is locally encoded by Set1/COMPASS on certain actively transcribed genes and that this protective role is most prominent at heterochromatin islands, small domains interspersed in euchromatin that regulate cell fate specifiers. Interestingly, this effect can be gene orientation dependent. Sensitivity to invasion by heterochromatin, surprisingly, is not dependent on Set1 altering overall gene expression levels. At least two independent pathways direct this Set1 activity{\textendash}inhibition of catalysis by Suv39/Clr4 and disruption of nucleosome stability. Taken together, these results describe a mechanism for spatial encoding of euchromatic signals that repel heterochromatin invasion.}, URL = {https://www.biorxiv.org/content/early/2019/05/08/630970}, eprint = {https://www.biorxiv.org/content/early/2019/05/08/630970.full.pdf}, journal = {bioRxiv} }