RT Journal Article
SR Electronic
T1 D-alanylation of Teichoic Acids in <em>Bacilli</em> impedes the immune sensing of peptidoglycan in <em>Drosophila</em>
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 631523
DO 10.1101/631523
A1 Zaynoun Attieh
A1 Mireille Kallassy Awad
A1 Agnès Rejasse
A1 Pascal Courtin
A1 Ivo Gomperts Boneca
A1 Marie-Pierre Chapot-Chartier
A1 Vincent Sanchis Borja
A1 Laure El Chamy
YR 2019
UL http://biorxiv.org/content/early/2019/05/08/631523.abstract
AB Modification of cell wall components is a prominent mean for pathogens to hinder host immune defenses. Here, using the Drosophila model, we aimed at characterizing the role of D-alanine esterification of teichoic acids (TAs) in the resistance of Bacillus thuringiensis to host defenses in vivo. We show that, by preventing cationic antimicrobial effectors-mediated bacterial lysis, this cell wall modification also limits the release of peptidoglycan immunostimulatory fragments thus impeding their sensing and the subsequent induction of the IMD- NF-κB pathway. Interestingly, we show that this strategy is also adopted by Lactobacillus plantarum, a bona fide commensal, to fine-tune its immunomodulatory potential in the Drosophila gut. Markedly, we show that the D-alanylation of TAs is essential for the resistance of L. plantarum to gut lysozyme. Altogether our data shed light on the mechanism underlying the persistence and the low immunostimulatory potential of L. plantarum in the Drosophila gut.