@article {McCallan235184, author = {L. McCallan and C. Brooks and C. Couzens and F. Young and A.W. Byrne and J. McNair}, title = {Performance of serological antibody tests for bovine tuberculosis in cattle from infected herds in Northern Ireland}, elocation-id = {235184}, year = {2017}, doi = {10.1101/235184}, publisher = {Cold Spring Harbor Laboratory}, abstract = {The ability to accurately identify infected hosts is the cornerstone of effective disease control and eradication programs. In the case of bovine tuberculosis, caused by infection with the pathogen Mycobacterium bovis, accurately identifying infected individual animals has been challenging as all available tests exhibit less than 100\% discriminatory ability. Here we assess the utility of three serological tests and assess their performance relative to skin test (Single Intradermal Comparative Cervical Tuberculin; SICCT), gamma-interferon (IFNγ) and post-mortem results in a Northern Ireland setting. Furthermore, we describe a case-study where one test was used in conjunction with statutory testing.Serological tests using samples taken prior to SICCT disclosed low proportions of animals as test positive (mean 3\% positive), despite the cohort having high proportions with positive SICCT test under standard interpretation (121/921; 13\%) or IFNγ (365/922; 40\%) results. Furthermore, for animals with a post-mortem record (n=286), there was a high proportion with TB visible lesions (27\%) or with laboratory confirmed infection (25\%). As a result, apparent sensitivities within this cohort was very low (<=15\%), however the tests succeeded in achieving very high specificities (96-100\%). During the case-study, 7/670 (1.04\%) samples from SICCT negative animals from a large chronically infected herd were serology positive, with a further 10 animals being borderline positive (17/670; 2.54\%). 9/17 of these animals were voluntarily removed, none of which were found to be infected (-lesions/-bacteriology) post-mortem; 1 serology test negative animal was subsequently lesion+ and M bovis confirmed at slaughter.Importance Eradication of bovine tuberculosis (bTB; caused by Mycobacterium bovis) has remained elusive in a number of countries despite long-term coordinated test and cull programs. This can partially be explained by the limitations of available statutory tests; therefore supplementary test platforms that identify additional infected animals would be of significant utility. Overall, during our study three serological tests did not disclose a high proportion of animals as infected in high-risk cattle herds, and exhibited limited ability to disclose animals that were positive to the statutory skin test, the gamma interferon test (IFNγ), or were post-mortem confirmed with M. bovis. These serological tests could be used in a supplementary fashion to the statutory tests in particular circumstances; but may be of limited advantage where parallel use of IFNγ and skin testing is performed, as these tests together tended to disclose the majority of animals with post-mortem evidence of infection in our study cohort.}, URL = {https://www.biorxiv.org/content/early/2017/12/18/235184}, eprint = {https://www.biorxiv.org/content/early/2017/12/18/235184.full.pdf}, journal = {bioRxiv} }