RT Journal Article SR Electronic T1 A pathway-centric view of spatial proximity in the 3D nucleome across cell lines JF bioRxiv FD Cold Spring Harbor Laboratory SP 027045 DO 10.1101/027045 A1 Karathia, Hiren A1 Kingsford, Carl A1 Girvan, Michelle A1 Hannenhalli, Sridhar YR 2015 UL http://biorxiv.org/content/early/2015/09/17/027045.abstract AB Spatial organization of the genome is critical for condition-specific gene expression. Previous studies have shown that functionally related genes tend to be spatially proximal. However, these studies have not been extended to multiple human cell types, and the extent to which context-specific spatial proximity of a pathway is related to its context-specific activity is not known. We report the first pathway-centric analyses of spatial proximity in six human cell lines. We find that spatial proximity of genes in a pathway tends to be context-specific, in a manner consistent with the pathway’s context-specific expression and function; housekeeping genes are ubiquitously proximal to each other, and cancer-related pathways such as p53 signaling are uniquely proximal in hESC. Intriguingly, we find a correlation between the spatial proximity of genes and interactions of their protein products, even after accounting for the propensity of co-pathway proteins to interact. Related pathways are also often spatially proximal to one another, and housekeeping genes tend to be proximal to several other pathways suggesting their coordinating role. Further, the spatially proximal genes in a pathway tend to be the drivers of the pathway activity and are enriched for transcription, splicing and transport functions. Overall, our analyses reveal a pathway-centric organization of the 3D nucleome whereby functionally related and interacting genes, particularly the initial drivers of pathway activity, but also genes across multiple related pathways, are in spatial proximity in a context-specific way. Our results provide further insights into the role of differential spatial organization in cell type-specific pathway activity.