RT Journal Article
SR Electronic
T1 Single-cell transcriptomes of the aging human skin reveal loss of fibroblast priming
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 633131
DO 10.1101/633131
A1 Llorenç Solé-Boldo
A1 Günter Raddatz
A1 Sabrina Schütz
A1 Jan-Philipp Mallm
A1 Karsten Rippe
A1 Anke S. Lonsdorf
A1 Manuel Rodríguez-Paredes
A1 Frank Lyko
YR 2019
UL http://biorxiv.org/content/early/2019/05/09/633131.abstract
AB Fibroblasts are the main dermal cell type and are essential for the architecture and function of human skin. Important differences have been described between fibroblasts localized in distinct dermal layers, and these cells are also known to perform varied functions. However, this phenomenon has not been analyzed comprehensively yet. Here we have used single-cell RNA sequencing to analyze >15,000 cells from a sun-protected area in young and old donors. Our results define four main fibroblast subpopulations that can be spatially localized and functionally distinguished. Importantly, intrinsic aging reduces this fibroblast ‘priming’, generates distinct expression patterns of skin aging-associated genes, and substantially reduces the interactions of dermal fibroblasts with other skin cell types. Our work thus provides comprehensive evidence for a functional specialization of human dermal fibroblasts and suggests that the age-related loss of fibroblast priming contributes to human skin aging.