RT Journal Article
SR Electronic
T1 A simple bioreactor-based method to generate kidney organoids from pluripotent stem cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 237644
DO 10.1101/237644
A1 Aneta Przepiorski
A1 Veronika Sander
A1 Tracy Tran
A1 Jennifer A. Hollywood
A1 Brie Sorrenson
A1 Jen-Hsing Shih
A1 Ernst J. Wolvetang
A1 Andrew P. McMahon
A1 Teresa M. Holm
A1 Alan J. Davidson
YR 2017
UL http://biorxiv.org/content/early/2017/12/20/237644.abstract
AB Kidney organoids generated from human pluripotent stem cells have the potential to revolutionize how kidney development and injury are studied. Current protocols are technically complex and suffer from poor reproducibility and high reagent costs restricting scalability. To overcome these issues, we have established a simple, inexpensive and robust method to grow kidney organoids in bulk from human induced pluripotent stem cells. Our organoids develop tubular structures by day (d) 8 and show optimal tissue morphology at d14. A comparison with fetal human kidney suggests that d14 organoid renal structures most closely resemble ‘capillary loop’ stage nephrons. We show that deletion of HNF1B, a transcription factor linked to congenital kidney defects, interferes with tubulogenesis, validating our experimental system for studying renal developmental biology. Taken together, our protocol provides a fast, efficient and cost-effective method for generating large quantities of human fetal kidney tissue, enabling the study of normal and aberrant human renal development.