PT  - JOURNAL ARTICLE
AU  - Hugo Girão
AU  - Naoyuki Okada
AU  - Ana C. Figueiredo
AU  - Zaira Garcia
AU  - Tatiana Moutinho-Santos
AU  - Jorge Azevedo
AU  - Sandra Macedo-Ribeiro
AU  - Ikuko Hayashi
AU  - Helder Maiato
TI  - CLASP2 lattice-binding near microtubule plus ends stabilizes kinetochore attachments
AID  - 10.1101/634907
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 634907
4099  - http://biorxiv.org/content/early/2019/05/10/634907.short
4100  - http://biorxiv.org/content/early/2019/05/10/634907.full
AB  - The fine regulation of kinetochore microtubule dynamics during mitosis ensures proper chromosome segregation by promoting error correction and spindle assembly checkpoint (SAC) satisfaction. CLASPs are widely conserved microtubule plus-end-tracking proteins that regulate microtubule dynamics throughout the cell cycle and independently localize to kinetochores during mitosis. Thus, CLASPs are ideally positioned to regulate kinetochore microtubule dynamics, but the underlying molecular mechanism remains unknown. Here we found that human CLASP2 can dimerize through its C-terminal kinetochore-targeting domain, but kinetochore localization was independent of dimerization. CLASP2 kinetochore localization, microtubule plus-end-tracking and microtubule lattice binding through TOG2 and TOG3 (but not TOG1) domains, independently sustained normal spindle length, timely SAC satisfaction, chromosome congression and faithful segregation. Measurements of kinetochore microtubule half-life in living cells expressing RNAi-resistant mutants revealed that CLASP2 kinetochore localization, microtubule plus-end-tracking and lattice binding cooperatively modulate kinetochore microtubule stability during mitosis. Thus, CLASP2 regulates kinetochore microtubule dynamics by integrating distinctive microtubule-binding properties at the kinetochore-microtubule interface to ensure chromosome segregation fidelity.