PT  - JOURNAL ARTICLE
AU  - Gregory R. Johnson
AU  - Rory M. Donovan-Maiye
AU  - Mary M. Maleckar
TI  - Building a 3D Integrated Cell
AID  - 10.1101/238378
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 238378
4099  - http://biorxiv.org/content/early/2017/12/21/238378.short
4100  - http://biorxiv.org/content/early/2017/12/21/238378.full
AB  - We present a conditional generative model for learning variation in cell and nuclear morphology and predicting the location of subcellular structures from 3D microscopy images. The model generalizes well to a wide array of structures and allows for a probabilistic interpretation of cell and nuclear morphology and structure localization from fluorescence images. We demonstrate the effectiveness of the approach by producing and evaluating photo-realistic 3D cell images using the generative model, and show that the conditional nature of the model provides the ability to predict the localization of unobserved structures, given cell and nuclear morphology. We additionally explore the model’s utility in a number of applications, including cellular integration from multiple experiments and exploration of variation in structure localization. Finally, we discuss the model in the context of foundational and contemporary work and suggest forthcoming extensions.