RT Journal Article SR Electronic T1 Transcription-dependent spreading of canonical yeast GATA factor across the body of highly expressed genes JF bioRxiv FD Cold Spring Harbor Laboratory SP 238550 DO 10.1101/238550 A1 Aria Ronsmans A1 Maxime Wéry A1 Camille Gautier A1 Marc Descrimes A1 Evelyne Dubois A1 Antonin Morillon A1 Isabelle Georis YR 2017 UL http://biorxiv.org/content/early/2017/12/22/238550.abstract AB GATA transcription factors are highly conserved among eukaryotes and play roles in transcription of genes implicated in cancer progression and hematopoiesis. However, although their consensus binding sites have been well defined in vitro, the in vivo selectivity for recognition by GATA factors remains poorly characterized. Using ChIP-Seq, we identified the Dal80 GATA factor targets in yeast. Our data reveal Dal80 binding to a large set of promoters, sometimes independently of GATA sites. Strikingly, Dal80 was also detected across the body of promoter-bound genes, correlating with high, Dal80-sensitive expression. Mechanistic single-gene experiments showed that Dal80 spreading across gene bodies is independent of intragenic GATA sites but requires transcription elongation. Consistently, Dal80 co-purified with the post-initiation form of RNA Polymerase II. Our work suggests that GATA factors could play dual, synergistic roles during transcription initiation and elongation, promoting efficient remodeling of the gene expression program in response to environmental changes.