PT  - JOURNAL ARTICLE
AU  - AC Hepburn
AU  - EL Curry
AU  - M Moad
AU  - RE Steele
AU  - OE Franco
AU  - L Wilson
AU  - P Singh
AU  - SE Crawford
AU  - Luke Gaughan
AU  - IG Mills
AU  - SW Hayward
AU  - CN Robson
AU  - R Heer
TI  - High-throughput propagation of human prostate tissue from induced-pluripotent stem cells
AID  - 10.1101/637876
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 637876
4099  - http://biorxiv.org/content/early/2019/05/14/637876.short
4100  - http://biorxiv.org/content/early/2019/05/14/637876.full
AB  - Primary culture of human prostate organoids is slow, inefficient and laborious. To overcome this, we demonstrate a new high-throughput model where rapidly proliferating and easily handled induced pluripotent stem cells, for the first time, enable generation of human prostate tissue in vivo and in vitro. Using a co-culture technique with urogenital sinus mesenchyme, we recapitulated the in situ prostate histology, including the stromal compartment and the full spectrum of epithelial differentiation. This approach overcomes major limitations in primary cultures of human prostate stem, luminal and neuroendocrine cells, as well as the stromal microenvironment. These models provide new opportunities to study prostate development, homeostasis and disease.