RT Journal Article SR Electronic T1 Cryo-EM structure of the MinCD copolymeric filament from Pseudomonas aeruginosa at 3.1 Å resolution JF bioRxiv FD Cold Spring Harbor Laboratory SP 638619 DO 10.1101/638619 A1 Andrzej Szewczak-Harris A1 James Wagstaff A1 Jan Löwe YR 2019 UL http://biorxiv.org/content/early/2019/05/15/638619.abstract AB Positioning of the division site in many bacterial species relies on the MinCDE system, which prevents the cytokinetic Z-ring from assembling anywhere but the mid-cell, through an oscillatory diffusion-reaction mechanism. MinD dimers bind to membranes and, via their partner MinC, inhibit the polymerisation of cell division protein FtsZ into the Z-ring. MinC and MinD form polymeric assemblies in solution and on cell membranes. Here, we report the high-resolution cryo-EM structure of the copolymeric filaments of Pseudomonas aeruginosa MinCD. The filaments consist of three protofilaments made of alternating MinC and MinD dimers. The MinCD protofilaments are almost completely straight and assemble as single protofilaments on lipid membranes, which we also visualised by cryo-EM.Cryo-EMelectron cryo-microscopyFSCFourier shell correlationIPTGisopropyl β-d-1-thiogalactopyranosideWACAWalker A cytoskeletal ATPaseTEMtransmission electron microscopeMTSmembrane targeting sequence