RT Journal Article
SR Electronic
T1 Role of the Visual Experience-Dependent Nascent Proteome in Neuronal Plasticity
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 240119
DO 10.1101/240119
A1 Han-Hsuan Liu
A1 Daniel B. McClatchy
A1 Lucio Schiapparelli
A1 Wanhua Shen
A1 John R. Yates III
A1 Hollis T. Cline
YR 2017
UL http://biorxiv.org/content/early/2017/12/27/240119.abstract
AB Experience-dependent synaptic plasticity refines brain circuits during development. To identify novel protein synthesis-dependent mechanisms contributing to experience-dependent plasticity, we conducted a quantitative proteomic screen of the nascent proteome in response to visual experience in Xenopus optic tectum using bio-orthogonal metabolic labeling (BONCAT). We identified 83 differentially synthesized candidate plasticity proteins (CPPs). The CPPs form strongly interconnected networks and are annotated to a variety of biological functions, including RNA splicing, protein translation, and chromatin remodeling. Functional analysis of select CPPs revealed the requirement for eukaryotic initiation factor 3 subunit A (eIF3A), fused in sarcoma (FUS), and ribosomal protein s17 (RPS17) in experience-dependent structural plasticity in tectal neurons and behavioral plasticity in tadpoles. These results demonstrate that the nascent proteome is dynamic in response to visual experience and that de novo synthesis of machinery that regulates RNA splicing and protein translation is required for experience-dependent plasticity.The nascent proteome changes dynamically in response to visual experienceNewly synthesized candidate plasticity proteins were identified using MS/MSVisual experience induces de novo synthesis of synaptic and cytoskeletal proteinsSynthesis of RNA splicing and translation machinery is required for plasticityeTOC Blurb Liu et al. show that the nascent proteome changes dynamically in response to plasticity-inducing visual experience. Functional analysis reveals that visual experience-dependent synthesis of RNA splicing and protein translation machinery is required for plasticity.