PT  - JOURNAL ARTICLE
AU  - Christine E. Cucinotta
AU  - A. Elizabeth Hildreth
AU  - Brendan M. McShane
AU  - Margaret K. Shirra
AU  - Karen M. Arndt
TI  - The nucleosome acidic patch directly interacts with subunits of the Paf1 and FACT complexes and controls chromatin architecture <em>in vivo</em>
AID  - 10.1101/637223
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 637223
4099  - http://biorxiv.org/content/early/2019/05/17/637223.short
4100  - http://biorxiv.org/content/early/2019/05/17/637223.full
AB  - The nucleosome core regulates DNA-templated processes through the highly conserved nucleosome acidic patch. While structural and biochemical studies have shown that the acidic patch controls chromatin factor binding and activity, few studies have elucidated its functions in vivo. We employed site-specific crosslinking to identify proteins that directly bind the acidic patch in Saccharomyces cerevisiae and demonstrated crosslinking of histone H2A to Paf1 complex subunit Rtf1 and FACT subunit Spt16. Rtf1 bound to nucleosomes through its histone modification domain, supporting its role as a cofactor in H2B K123 ubiquitylation. An acidic patch mutant showed defects in nucleosome positioning and occupancy genome-wide. Our results provide new information on the chromatin engagement of two central players in transcription elongation and emphasize the importance of the nucleosome core as a hub for proteins that regulate chromatin during transcription.