TY - JOUR T1 - The maternal-fetal interface of successful pregnancies and impact of fetal sex using single cell sequencing JF - bioRxiv DO - 10.1101/641118 SP - 641118 AU - Tianyanxin Sun AU - Tania L. Gonzalez AU - Nan Deng AU - Rosemarie DiPentino AU - Ekaterina L. Clark AU - Bora Lee AU - Jie Tang AU - Yizhou Wang AU - Barry R. Stripp AU - Changfu Yao AU - Hsian-Rong Tseng AU - S. Ananth Karumanchi AU - Alexander F. Koeppel AU - Stephen D. Turner AU - Charles R. Farber AU - Stephen S. Rich AU - Erica T. Wang AU - John Williams III AU - Margareta D. Pisarska Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/05/18/641118.abstract N2 - The first trimester is a critical window of maternal-fetal communication for pregnancy. Therefore, we characterized crosstalk in ongoing human pregnancies at 11-13 weeks gestation. RNA-sequencing of matched maternal decidua and placenta identified 818 receptors and 3502 ligands, including 126 differentially expressed receptor-ligand pairs. Using single cell RNA-sequencing to further dissect placenta heterogeneity, we identified five major cell types (trophoblasts, stromal cells, hofbauer cells, antigen presenting cells and endothelial cells) with unique crosstalk at the maternal-fetal interface. We identified seven unique trophoblast subclusters, including new subtypes that transition into the terminal cell types, extra-villous trophoblasts and syncytiotrophoblasts. As fetal sex impacts pregnancy, we analyzed sex differences in each cell type and identified differences in immune cell function. TGFβ1, β-estradiol, and dihydrotestosterone emerge as upstream regulators of sexually dimorphic genes in a cell type specific manner. Thus, the fetal contribution at the maternal-fetal interface is cell and sex specific. ER -