RT Journal Article
SR Electronic
T1 Fcrl5 and T-bet define influenza-specific memory B cells that predict long-lived antibody responses
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 643973
DO 10.1101/643973
A1 Anoma Nellore
A1 Christopher D. Scharer
A1 Rodney G. King
A1 Christopher M. Tipton
A1 Esther Zumaquero
A1 Christopher Fucile
A1 Betty Mousseau
A1 John E. Bradley
A1 Kevin Macon
A1 Tian Mi
A1 Paul A. Goepfert
A1 John F. Kearney
A1 Jeremy M. Boss
A1 Troy D. Randall
A1 Ignacio Sanz
A1 Alexander Rosenberg
A1 Frances E. Lund
YR 2019
UL http://biorxiv.org/content/early/2019/05/20/643973.abstract
AB Early surrogates for long-lived immunity after inactivated influenza vaccination (IIV) are lacking. Antigen-specific memory B cells (Bmem) after IIV have been recently identified. We show that the antigen-specific Bmem compartment after IIV is heterogenous and comprises a clonotypically and transcriptionally distinct T-bethi subset that persists in circulation over time after vaccination and exclusively correlates with the long-lived antibody response. We demonstrate that this subset has an effector memory transcriptome and is epigenetically remodeled to facilitate intracellular immunoglobulin production. Finally, via clonal sharing, we show an enriched in vivo ontologic relationship between the secondary plasmablast response that develops after vaccine boost and the T-bethi fraction of the flu-specific Bmem response that forms after initial prime. Collectively, our data identify a novel biomarker of durable humoral immunity after influenza vaccination.