PT - JOURNAL ARTICLE AU - Alperen Guver AU - Nafetalai Fifita AU - Peker Milas AU - Michael Straker AU - Michael Guy AU - Kara Green AU - Taha Yildirim AU - Ilyas Unlu AU - Veysel Yigit AU - Birol Ozturk TI - A low-cost and high-precision scanning electrochemical microscope built with open source tools AID - 10.1101/645283 DP - 2019 Jan 01 TA - bioRxiv PG - 645283 4099 - http://biorxiv.org/content/early/2019/05/21/645283.short 4100 - http://biorxiv.org/content/early/2019/05/21/645283.full AB - A low-cost Scanning Electrochemical Microscope (SECM) was built with a 0.6 pA current measurement capability potentiostat and submicron resolution motorized stage, using open source software and hardware tools. The high performance potentiostat with a Python graphical user interface was built based on an open source project. Arduino boards, stepper motors, a manual XY micromanipulator stage, 3D printed couplers and gears were used in building the motorized stage. An open source motor control software was used for moving the motorized stage with high precision. An inverted microscope was utilized for viewing a standard microelectrode while scanning. The setup was tested in the formation of a map of electrochemical signals from an array of pores on a parafilm membrane. As the setup will be used in future biosensing experiments, DNA hybridization detection experiments were also performed with the setup.