RT Journal Article SR Electronic T1 Chromatin Functional States Correlate with HIV Latency Reversal in Infected Primary CD4+ T cells JF bioRxiv FD Cold Spring Harbor Laboratory SP 242958 DO 10.1101/242958 A1 Emilie Battivelli A1 Matthew S. Dahabieh A1 Mohamed Abdel-Mohsen A1 J. Peter Svensson A1 Israel Tojal Da Silva A1 Lilian B. Cohn A1 Andrea Gramatica A1 Steven Deeks A1 Warner Greene A1 Satish K. Pillai A1 Eric Verdin YR 2018 UL http://biorxiv.org/content/early/2018/01/04/242958.abstract AB Human immunodeficiency virus (HIV) infection cannot be cured due to a small reservoir of latently infected CD4+ T cells in treated patients. The “shock and kill” approach proposes to eliminate the reservoir by inducing its activation and the direct or indirect killing of infected cells. Current latency reversing agents (LRAs) do not reduce the viral reservoir in treated patients.We use a novel dual-fluorescent HIV reporter to identify and purify latent cells, and to determine the fraction of latent cells that undergo viral reactivation after infection of primary CD4+ T cells. Unexpectedly, LRAs reactivate less than 5% of latent proviruses. Analysis of HIV integration sites from induced and non-induced latent populations reveals distinct provirus integration sites between these two populations in terms of chromatin functional states.These findings challenge “shock and kill”, and suggest the need of more potent LRAs in combination with immunomodulatory approaches to eradicate HIV reservoir.