RT Journal Article
SR Electronic
T1 Mitochondrial dynamics quantitatively revealed by STED nanoscopy with an enhanced squaraine variant probe
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 646117
DO 10.1101/646117
A1 Xusan Yang
A1 Zhigang Yang
A1 Ying He
A1 Chunyan Shan
A1 Wei Yan
A1 Zhaoyang Wu
A1 Peiyuan Chai
A1 Junlin Teng
A1 Junle Qu
A1 Peng Xi
YR 2019
UL http://biorxiv.org/content/early/2019/05/22/646117.abstract
AB Mitochondria play a critical role in generating energy to support the entire lifecycle of biological cells, yet it is still unclear how their morphological structures evolve to regulate their functionality. Conventional fluorescence microscopy can only provide ∼300 nm resolution, which is insufficient to visualize mitochondrial cristae. Here, we developed an enhanced squaraine variant dye (MitoESq-635) to study the dynamic structures of mitochondrial cristae in live cells at superresolution. The low saturation intensity and high photostability make it ideal for long-term, high-resolution STED nanoscopy. We demonstrate the time-lapsed imaging of the mitochondrial inner membrane over 50 minutes in living HeLa cells at 35.2 nm resolution for the first time. The forms of the cristae during mitochondrial fusion and fission can be clearly resolved. Our study demonstrates the emerging capability of optical STED nanoscopy to investigate intracellular physiological processes at nanoscale resolution for long periods of time with minimal phototoxicity.