RT Journal Article
SR Electronic
T1 PICKLE recruits RETINOBLASTOMA RELATED 1 to Control Lateral Root Formation in <em>Arabidopsis</em>
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 643122
DO 10.1101/643122
A1 Krisztina Ötvös
A1 Pál Miskolczi
A1 Peter Marhavý
A1 Alfredo Cruz-Ramírez
A1 Eva Benková
A1 Stéphanie Robert
A1 László Bakó
YR 2019
UL http://biorxiv.org/content/early/2019/05/22/643122.abstract
AB Lateral root (LR) formation is an example of plant post-embryonic organogenesis event. LRs are issued from non-dividing cells entering consecutive steps of formative divisions, proliferation and elongation. The chromatin remodeling protein PICKLE negatively regulates auxin-mediated LR formation through a mechanism that is not yet known. Here we show that PICKLE interacts with RETINOBLASTOMA-RELATED 1 (RBR1) to repress the LATERAL ORGAN BOUNDARIES-DOMAIN 16 (LBD16) promoter activity. Since LBD16 function is required for the formative division of LR founder cells, repression mediated by the PKL-RBR1 complex negatively regulates formative division and LR formation. Inhibition of LR formation by PKL-RBR1 is counteracted by auxin indicating that in addition to auxin-mediated transcriptional responses, the fine-tuned process of LR formation is also controlled at the chromatin level in an auxin-signaling dependent manner.