PT  - JOURNAL ARTICLE
AU  - Ling Chu
AU  - Jonathan Tyson
AU  - Juliana E. Shaw
AU  - Felix Rivera-Molina
AU  - Anthony J. Koleske
AU  - Alanna Schepartz
AU  - Derek K. Toomre
TI  - Two-color nanoscopy of organelles for extended times with HIDE probes
AID  - 10.1101/647065
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 647065
4099  - http://biorxiv.org/content/early/2019/05/22/647065.short
4100  - http://biorxiv.org/content/early/2019/05/22/647065.full
AB  - Performing multi-color nanoscopy for extended times is challenging due to the rapid photobleaching rate of most fluorophores. Here we describe a new fluorophore (Yale-595) and a bio-orthogonal labeling strategy that enables both super-resolution (STED) and 3D confocal imaging of two organelles simultaneously for extended times using high-density environmentally sensitive (HIDE) probes. Because HIDE probes are small, cell-permeant molecules, they can visualize organelle pairs (ER + mitochondria, ER + plasma membrane) in hard-to-transfect cell lines at super-resolution for up to 7 minutes. The extended time domain possible using these new tools reveal novel dynamic nanoscale targeting between organelles.