PT  - JOURNAL ARTICLE
AU  - Mahringer, David
AU  - Petersen, Anders V.
AU  - Fiser, Aris
AU  - Okuno, Hiroyuki
AU  - Bito, Haruhiko
AU  - Perrier, Jean-François
AU  - Keller, Georg B.
TI  - Expression of c-Fos and Arc in hippocampal region CA1 marks neurons that exhibit learning-related activity changes
AID  - 10.1101/644526
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 644526
4099  - http://biorxiv.org/content/early/2019/05/23/644526.short
4100  - http://biorxiv.org/content/early/2019/05/23/644526.full
AB  - Immediate early genes (IEGs) are transcribed in response to neural activity and necessary for many forms of plasticity. However, the dynamics of their expression during learning, as well as their relationship to neural activity, remain unclear. Here we used two-photon imaging in transgenic mice that express a GFP-tagged variant of Arc or c-Fos and a red-shifted calcium indicator to measure learning-related changes in IEG expression levels and neural activity in hippocampal region CA1 as mice learned to perform a two-alternative forced choice task. Neural activity levels correlated positively with IEG expression levels in vivo. In addition, we found that with learning, a subset of neurons in CA1 increased their responses to the reward-predicting cue, and IEG expression levels early in learning were selectively increased in neurons that would exhibit the strongest learning-related changes. Our findings are consistent with an interpretation of IEG expression levels as markers for experience dependent plasticity.