RT Journal Article
SR Electronic
T1 Root development is maintained by specific bacteria-bacteria interactions within a complex microbiome
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 645655
DO 10.1101/645655
A1 Omri M. Finkel
A1 Isai Salas-González
A1 Gabriel Castrillo
A1 Theresa F. Law
A1 Jonathan M. Conway
A1 Corbin D. Jones
A1 Jeffery L. Dangl
YR 2019
UL http://biorxiv.org/content/early/2019/05/23/645655.abstract
AB Plants grow within a complex web of species interacting with each other and with the plant via a wide repertoire of chemical signals. To model plant-microbe-microbe-environment interactions, we inoculated seedlings with a defined 185-member bacterial synthetic community (SynCom), and manipulated the abiotic environment to enable classification of the SynCom to modules of co-occurring strains. We deconstructed the SynCom based on these modules, identifying a single bacterial genus, Variovorax, which reverts phenotypic effects on root development induced by a wide diversity of bacterial strains and by the entire 185-member community. Variovorax use mechanisms related to auxin and ethylene manipulation to balance this ecologically realistic root community’s effects on root development. We demonstrate metabolic signal interference within a complex model community, defining Variovorax as determinants of bacteria-plant communication networks.