RT Journal Article
SR Electronic
T1 Methionine metabolism influences the genomic architecture of H3K4me3 with the link to gene expression encoded in peak width
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 243196
DO 10.1101/243196
A1 Ziwei Dai
A1 Samantha J. Mentch
A1 Xia Gao
A1 Sailendra N. Nichenametla
A1 Jason W. Locasale
YR 2018
UL http://biorxiv.org/content/early/2018/01/05/243196.abstract
AB Nutrition and metabolism are known to influence chromatin biology and epigenetics by modifying the levels of post-translational modifications on histones, yet how changes in nutrient availability influence specific aspects of genomic architecture and connect to gene expression is unknown. To investigate this question we considered, as a model, the metabolically-driven dynamics of H3K4me3, a histone methylation mark that is known to encode information about active transcription, cell identity, and tumor suppression. We analyzed the genome-wide changes in H3K4me3 and gene expression in response to alterations in methionine availability under conditions that are known to affect the global levels of histone methylation in both normal rodent physiology and in human cancer cells. Surprisingly, we found that the location of H3K4me3 peaks at specific genomic loci was largely preserved under conditions of methionine restriction. However, upon examining different geometrical features of peak shape, it was found that the response of H3K4me3 peak width encoded almost all aspects of H3K4me3 biology including changes in expression levels, and the presence of cell identity and cancer associated genes. These findings reveal simple yet new and profound principles for how nutrient availability modulates specific aspects of chromatin dynamics to mediate key biological features.