PT - JOURNAL ARTICLE AU - Catherine M. Flanley AU - Marcelo Ramalho-Ortigao AU - Iliano V. Coutinho-Abreu AU - Rami Mukbel AU - Hanafi A. Hanafi AU - Shabaan S. El-Hossary AU - Emadeldin Y. Fawaz AU - David F. Hoel AU - Alexander W. Bray AU - Gwen Stayback AU - Douglas A. Shoue AU - Shaden Kamhawi AU - Scott Emrich AU - Mary Ann McDowell TI - <em>Phlebotomus papatasi</em> sand fly salivary protein diversity and immune response potential in Egypt and Jordan populations AID - 10.1101/649517 DP - 2019 Jan 01 TA - bioRxiv PG - 649517 4099 - http://biorxiv.org/content/early/2019/05/24/649517.short 4100 - http://biorxiv.org/content/early/2019/05/24/649517.full AB - Phlebotomus papatasi sand flies inject their hosts with a myriad of pharmacologically active salivary proteins to assist with blood feeding and to modulate host defenses. These salivary proteins have been studied for their role in cutaneous leishmaniasis disease outcome with different salivary proteins attenuating or exacerbating lesion size. Studies have shown that while co-administered sand fly saliva exacerbates Leishmania major infections in naïve mice, animals pre-exposed to saliva are protected, with the infection attenuated via a delayed-type hypersensitivity immune reaction. These studies highlight the potential of the salivary components to be used as a vaccine. One protein in particular, P. papatasi salivary protein 15 (PpSP15) has been intensively studied because of its ability to protect mice against Le. major challenge. The number of antigenic molecules included in vaccines is restricted thus emphasizing the role of population genetics to identify molecules, like PpSP15, that are functionally significant, conserved across populations and do not experience selection. Three distinct ecotope study sites, one in Egypt (Aswan) and two in Jordan (Swaimeh and Malka), were chosen based on their elevation, rainfall, vegetation, differing reservoir species, and the presence or absence of Le. major. The objective of this work was to analyze the genetic variability of nine of the most abundantly expressed salivary proteins including PpSP12, PpSP14, PpSP28, PpSP29, PpSP30, PpSP32, PpSP36, PpSP42, and PpSP44 and to predict their ability to elicit an immune response. Two proteins, PpSP12 and PpSP14, demonstrated low genetic variability across the three sand fly populations represented in this study, with multiple predicted MHCII epitope binding sites, identified by alleles present in the human populations from the study sites. The other seven salivary proteins revealed greater allelic variation across the same sand fly populations indicating that their use as vaccine targets may prove to be challenging.