%0 Journal Article
%A Baker, Cody
%A Froudarakis, Emmanouil
%A Yatsenko, Dimitri
%A Tolias, Andreas S.
%A Rosenbaum, Robert
%T Inference of Synaptic Connectivity and External Variability in Neural Microcircuits
%D 2019
%R 10.1101/650069
%J bioRxiv
%P 650069
%X A major goal in neuroscience is to estimate neural connectivity from large scale extracellular recordings of neural activity in vivo. This is challenging in part because any such activity is modulated by the unmeasured external synaptic input to the network, known as the common input problem. Many different measures of functional connectivity have been proposed in the literature, but their direct relationship to synaptic connectivity is often assumed or ignored. For in vivo data, measurements of this relationship would require a knowledge of ground truth connectivity, which is nearly always unavailable. Instead, many studies use in silico simulations as benchmarks for investigation, but such approaches necessarily rely upon a variety of simplifying assumptions about the simulated network and can depend on numerous simulation parameters. We combine neuronal network simulations, mathematical analysis, and calcium imaging data to address the question of when and how functional connectivity, synaptic connectivity, and latent external input variability can be untangled. We show numerically and analytically that, even though the precision matrix of recorded spiking activity does not uniquely determine synaptic connectivity, it is often closely related to synaptic connectivity in practice under various network models. This relation becomes more pronounced when the spatial structure of neuronal variability is considered jointly with precision.
%U https://www.biorxiv.org/content/biorxiv/early/2019/05/24/650069.full.pdf